A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.

V K Chaudhary, Janendra K. Batra, Maria Gallo, +3

Proceedings of the National Academy of Sciences

Microbial PathogensImmunologyMolecular BiologyEscherichia ColiRapid Method +16

A deleted variant of Bacillus anthracis protective antigen is non-toxic and blocks anthrax toxin action in vivo

Yogendra Singh, V K Chaudhary, Stephen H. Leppla

Journal of Biological Chemistry

Recombinant immunotoxins containing anti-Tac(Fv) and derivatives of Pseudomonas exotoxin produce complete regression in mice of an interleukin-2 receptor-expressing human carcinoma

RJ Kreitman, Pascal Bailon, V K Chaudhary, +2

Blood

The carboxyl-terminal end of protective antigen is required for receptor binding and anthrax toxin activity

Yogendra Singh, Kurt R. Klimpel, Conrad P. Quinn, +2

Journal of Biological Chemistry

Microbial ToxinAnthrax ToxinProtein FunctionAllergyAmino Acids +11

A recombinant single-chain immunotoxin composed of anti-Tac variable regions and a truncated diphtheria toxin.

V K Chaudhary, Maria Gallo, D J FitzGerald, +1

Proceedings of the National Academy of Sciences

ToxinologyAnti-tac Variable RegionsAmino AcidsLaboratory ImmunologyImmunology +16

Cytotoxic activity of an interleukin 6-Pseudomonas exotoxin fusion protein on human myeloma cells.

Clay B. Siegall, V K Chaudhary, David Fitzgerald, +1

Proceedings of the National Academy of Sciences

ImmunotoxicologyImmunologyPathologyCell DeathEscherichia Coli +16

Antitumor activity in mice of an immunotoxin made with anti-transferrin receptor and a recombinant form of Pseudomonas exotoxin.

Janendra K. Batra, Yoshihiro Jinno, V K Chaudhary, +4

Proceedings of the National Academy of Sciences

Antitumor ActivityImmunotoxicologyToxinologyImmunologyPathology +15

Recombinant toxins containing the variable domains of the anti-Tac monoclonal antibody to the interleukin-2 receptor kill malignant cells from patients with chronic lymphocytic leukemia

RJ Kreitman, V K Chaudhary, RW Kozak, +3

Blood

Mutagenesis of Pseudomonas exotoxin in identification of sequences responsible for the animal toxicity.

V K Chaudhary, Yoshihiro Jinno, Maria Gallo, +2

Journal of Biological Chemistry

Microbial ToxinProtein ExpressionAmino AcidsBiochemistryToxinology +13

Cytotoxic activity of a recombinant fusion protein between interleukin 4 and Pseudomonas exotoxin.

M Ogata, V K Chaudhary, David J. FitzGerald, +1

Proceedings of the National Academy of Sciences

Microbial PathogensImmunologyImmunologic MechanismCytotoxic ActivityBacterial Pathogens +17