Full-length transcriptome assembly from RNA-Seq data without a reference genome

Manfred Grabherr, Brian J. Haas, Moran Yassour, +17

Nature Biotechnology

Long-read SequencingFunctional GenomicsNatural SciencesGeneticsSequence Analysis +10

Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells

Alex K. Shalek, Rahul Satija, Xian Adiconis, +15

Nature

Immune CellsFunctional GenomicsSingle Cell SequencingMedicineImmunology +11

Ab initio reconstruction of cell type–specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs

Mitchell Guttman, Manuel Garber, Joshua Z. Levin, +10

Nature Biotechnology

Transcriptional RegulationGeneticsRna BiologyGene StructureMolecular Genetics +7

Comprehensive Classification of Retinal Bipolar Neurons by Single-Cell Transcriptomics

Karthik Shekhar, Sylvain W. Lapan, Irene E. Whitney, +11

Cell

OptogeneticsGanglion CellSingle Cell SequencingMedicineSingle-cell Genomics +4

Individual brain organoids reproducibly form cell diversity of the human cerebral cortex

Silvia Velasco, Amanda J. Kedaigle, Sean Simmons, +9

Nature

EpendymaBrain StructureNeuroanatomyMedicineHuman Cerebral Cortex +9

Systematic comparison of single-cell and single-nucleus RNA-sequencing methods

Jiarui Ding, Xian Adiconis, Sean Simmons, +18

Nature Biotechnology

BioinformaticsFunctional GenomicsSingle Cell SequencingMedicineGenetics +8

Comprehensive comparative analysis of strand-specific RNA sequencing methods

Joshua Z. Levin, Moran Yassour, Xian Adiconis, +5

Nature Methods

Comprehensive Comparative AnalysisSequencingFunctional GenomicsGeneticsSequence Analysis +6

Single-cell transcriptomic profiling of the aging mouse brain

Methodios Ximerakis, Scott Lipnick, Brendan T. Innes, +15

Nature Neuroscience

Single Cell SequencingMedicineSingle-cell GenomicsBiogerontologySingle-cell Analysis +2

Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes

Nicholas M. Tran, Karthik Shekhar, Irene E. Whitney, +14

Neuron

Ganglion CellNeuroregenerationRetinaOphthalmologyMedicine +6

Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples

Xian Adiconis, Diego Borges-Rivera, Rahul Satija, +11