Ved V. Topkar

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai, Zongli Zheng, Nathalie T. Nguyen et al. · Nature Biotechnology · 2014 · 2.2K citations · Full text

Off-target Effect, Genetics, Gene Editing +7

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai et al. · Nature · 2015 · 1.6K citations · Full text

Engineering, Synthetic Biology, Genetic Engineering +8

CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets

Shengdar Q. Tsai, Nathalie T. Nguyen, Jose Malagon-Lopez et al. · Nature Methods · 2017 · 791 citations · Full text

Vitro Screen, Natural Sciences, Genetics +8

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai et al. · Nature Biotechnology · 2015 · 616 citations · Full text

Pam Recognition, Off-target Effect, Engineering +10

Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics

Kathrin Leppek, Gun Woo Byeon, Wipapat Kladwang et al. · Nature Communications · 2022 · 340 citations · Full text