The B-Complex Vitamins of Musts and Wines As Microbial Growth Factors

Abstract

B-COMPLEX VITAMINS OF WINES isms.The soil was reperfused for 168 hours and B.O.D. determinations were made at the end of each 24 hour period up to 96 hours.The results of these studies are shown in Tables 2 and3.At the conclusion of the final perfusion the soil was plated in-S.N.W. agar.Colonies appeared in 48 hours and were predominantly of two types-non-pigmented, smooth pinpoint colonies and larger, rough colonies.Microscopic examination showed both types to be rods.No attempt was made to identify the microorganisms. SUMMARY AND CONCLUSIONSThe perfusion method is suggested as a procedure which may be used to enrich the soil with microorgan-isms which will reduce the B.O.D. of semi-chemical neutral sulfite waste by about 60 per cent.Plating of enriched soil after perfusion gave two predominant types of microorganisms.Evidence that soil microorganisms will utilize the S.N.W. is supported by the fact that these colonies grew on agar plates containing only S.N.W.