Journal of AOAC International · 2006 · 15 citations · 1 references
Life SciencesBiochemistryNutritional SciencesChromatographic AnalysisFood AnalysisNutritional BiochemistryAnalytical ChromatographyChromatographyLiquid ChromatographyVitamin AnalysisVitamin BiochemistryVitamin ChemistryBioanalytical ChromatographyHigh-performance Liquid ChromatographyHigh-performance Liquid Chromatography ColumnsHigh-performance Liquid Chromatography Method DevelopmentReversed-phase ColumnsVitamin Assay Development
A solid-phase extraction sample preparation procedure was developed for use with a high-performance liquid chromatography (HPLC) method for biotin analysis. The HPLC method used a reversed-phase C18 column; chromatography run time was 8.5 min. After eluting from the column, biotin went through postcolumn reaction to form a conjugate with streptavidin-fluorescein isothiocyanate, which was then detected by a fluorescence detector. This method was tested with infant formula, medical nutritional products, and vitamin premix samples.
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