The complement component C5 of the common carp (Cyprinus carpio): cDNA cloning of two distinct isotypes that differ in a functional site

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

The neighbor-joining method: a new method for reconstructing phylogenetic trees.

Naruya Saitou, M Nei · Molecular Biology and Evolution · 1987 · 60.5K citations · Full text

Life Sciences, Bioinformatics, Evolutionary Biology +17

A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples.

Piotr Chomczyński · PubMed · 1993 · 2.8K citations

Life Sciences, Biochemistry, Molecular Biology +17

Multiple sequence alignment with Clustal X

François Jeanmougin, Julie Thompson, Manolo Gouy et al. · Trends in Biochemical Sciences · 1998 · 2.7K citations

Life Sciences, Bioinformatics, Molecular Biology +5

Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio): presence of variants lacking the catalytic histidine

Miki Nakao, Junichi Mutsuro, Rie Obo et al. · European Journal of Immunology · 2000 · 127 citations

Life Sciences, Biochemistry, Molecular Biology +14