One-tube detection of <i>Salmonella</i> Typhimurium using LAMP and CRISPR-Cas12b

Abstract

Here, we have provided a novel one-step method for <i>Salmonella</i> Typhimurium detection in one pot by integrating the LAMP assay with the CRISPR/Cas12b system, offering significant advantages in terms of simplicity, speed, and accuracy.