The proteome of bacterial membrane vesicles in Escherichia coli—a time course comparison study in two different media

Abstract

Our study showed that the choice of medium and the duration of culturing significantly influence both <i>E. coli</i> bMV numbers and protein composition. Our TEM/Cryo-EM results demonstrated the presence of intact <i>E. coli</i> bMVs. Common <i>E. coli</i> proteins, including OmpA, GroEL, and ribosome proteins, can consistently be identified across all six tested growth conditions. Furthermore, our functional assays imply that bMVs isolated from the six groups retain their function and result in comparable cytokine induction.