ChemCatChem · 2023 · 28 citations · 25 references
EngineeringBioengineeringBiotechnologyBiocatalysisEnzymologyMetabolic EngineeringBiocatalyst DiscoveryBiocatalyst EngineeringMicrobial Natural Product BiosynthesisPathway EngineeringSecondary Metabolite BiosynthesisSynthetic Pathway ConstructionActive Site ResiduesCatechol-o-methyltransferase PathwaysRegioselective BiocatalysisRegioselective CatalysisRegioselective SynthesisRegioselectivityRegioselectivity Optimization
Abstract Directed evolution of the O ‐methyltransferase ZgOMT from Zooshikella ganghwensis focusing on active site residues resulted in highly regioselective biocatalysts (regioisomeric ratios up to 99 : 1) for the preparation of the taste active hesperetin dihydrochalcone and related compounds. These newly constructed enzyme variants provide an attractive synthesis route for para ‐methylation of catechol scaffolds, which is challenging to perform with high regioselectivity utilizing wild‐type O ‐methyltransferases.
25
ColabFold: making protein folding accessible to all
Milot Mirdita, Konstantin Schütze, Yoshitaka Moriwaki et al. · Nature Methods · 2022 · 9.7K citations · Full text
A new bioinformatics analysis tools framework at EMBL-EBI
M. Goujon, Hamish McWilliam, Weizhong Li et al. · Nucleic Acids Research · 2010 · 1.9K citations · Full text
Analysis Tool Web Services from the EMBL-EBI
Hamish McWilliam, Weizhong Li, Mahmut Uludağ et al. · Nucleic Acids Research · 2013 · 1.7K citations · Full text