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Occurrence of β2- Adrenergic Agonist Residues in Urine of Animal Meat Producers in Portugal
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1998
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Bovine Growth PromoterLaboratory Animal StudyAdrenal GlandBioanalysisBovine UrineToxicologyAnalytical ChemistryLiquid ChromatographyClinical ChemistryChromatographyHealth SciencesAnimal PhysiologyOxysterolBiochemistryChromatographic AnalysisPharmacologyAnimal SciencePhysiologyDecember 31,1993Animal Meat ProducersMetabolismMedicineMeat ScienceDrug Analysis
abstract Between January 1,1991, and December 31,1993, bovine urine was collected for analysis of β2-adrenergic agonist residues. A multiresidue method was developed that was capable of detecting clenbuterol, clenpenterol, clenproperol, mabuterol, mapenterol, bromobuterol, tulobuterol, and salbutamol through enzyme-linked immunosorbent assay, liquid chromatography (LC) with photodiode array detection, and gas chromatography with mass detection in electron impact mode with selective-ion monitoring. Among 1031 samples, 24 contained clenbuterol and 1 contained salbutamol. The results confirm that clenbuterol is the β2-adrenergic agonist most used as bovine growth promoter in Portugal.