The Epibiotic Microbiota of Wild Caribbean Sea Urchin Spines Is Species Specific

Abstract

Caribbean sea urchins are marine invertebrates that have experienced a decline over the years. Studies on sea urchins have focused primarily on the microbiome of the coelomic fluid or the gut microbiota. In this study, the epibiota community associated with four wild Caribbean sea urchin species, <i>Lytechinus variegatus</i>, <i>Echinometra lucunter</i>, <i>Tripneustes ventricosus</i>, and <i>Diadema antillarum</i>, was characterized for the first time. Using 57 sea urchin animal samples, we evaluated the influence of animal species, trophic niches, and geographical location on the composition of the epibiotic microbiota. We found significant differences in the bacterial biota among species and trophic niches, but not among geographical locations. <i>L. variegatus</i> exhibited the highest alpha diversity with high dominance of Fusobacteria, Planctomycetes, and Cyanobacteria, whereas <i>T. ventricosus</i> and <i>D. antillarum</i> were dominated by Firmicutes. <i>T. ventricosus</i> inhabiting the seagrass biotope dominated by <i>Thalassia testudinum</i> meadows had mostly <i>Endozoicomonas.</i> In contrast, samples located in the reef (dominated by corals and other reef builders) had a higher abundance of <i>Kistimonas</i> and <i>Photobacterium.</i> Our findings confirm that the epibiotic microbiota is species-specific, but also niche-dependent, revealing the trophic networks emerging from the organic matter being recycled in the seagrass and reef niches. As echinoids are important grazers of benthic communities, their microbiota will likely influence ecosystem processes.