Using QIIME to Analyze 16S rRNA Gene Sequences from Microbial Communities

TLDR

QIIME, an acronym for Quantitative Insights Into Microbial Ecology, is a software application used to analyze nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities. The protocols aim to guide users in installing QIIME on a single computer and applying it to analyze 16S sequence data from nine distinct microbial communities. The protocols detail the installation steps and subsequent analysis workflow for QIIME on a single computer. © 2012 John Wiley & Sons, Inc., Curr.

Abstract

Abstract QIIME (canonically pronounced “chime”) is a software application that performs microbial community analysis. It is an acronym for Quantitative Insights Into Microbial Ecology, and has been used to analyze and interpret nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities. The following protocols describe how to install QIIME on a single computer and use it to analyze microbial 16S sequence data from nine distinct microbial communities. Curr. Protoc. Microbiol . 27:1E.5.1‐1E.5.20. © 2012 by John Wiley & Sons, Inc.