PROSTATE SPECIFIC ORIGIN OF DIPEPTIDYLPEPTIDASE IV (CD-26) IN HUMAN SEMINAL PLASMA

Abstract

No AccessJournal of UrologyInvestigative Urology1 Nov 1998PROSTATE SPECIFIC ORIGIN OF DIPEPTIDYLPEPTIDASE IV (CD-26) IN HUMAN SEMINAL PLASMA MICHAEL J. WILSON, AMY R. RUHLAND, JON L. PRYOR, CESAR ERCOLE, AKHOURI A. SINHA, HUGH HENSLEIGH, KEITH W. KAYE, HUGH J.S. DAWKINS, NEIL F. WASSERMAN, PRATAP REDDY, and KHALIL AHMED MICHAEL J. WILSONMICHAEL J. WILSON More articles by this author , AMY R. RUHLANDAMY R. RUHLAND More articles by this author , JON L. PRYORJON L. PRYOR More articles by this author , CESAR ERCOLECESAR ERCOLE More articles by this author , AKHOURI A. SINHAAKHOURI A. SINHA More articles by this author , HUGH HENSLEIGHHUGH HENSLEIGH More articles by this author , KEITH W. KAYEKEITH W. KAYE More articles by this author , HUGH J.S. DAWKINSHUGH J.S. DAWKINS More articles by this author , NEIL F. WASSERMANNEIL F. WASSERMAN More articles by this author , PRATAP REDDYPRATAP REDDY More articles by this author , and KHALIL AHMEDKHALIL AHMED More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62441-8AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: A number of peptidases which can metabolize certain bioactive peptides and growth factors have been identified in seminal plasma. Our goal in this study was to determine molecular properties and the tissue source(s) for one of these peptidases, dipeptidylpeptidase IV (DPP IV), in human seminal plasma. Materials and Methods: We measured the activities of DPP IV with the dipeptide glycylprolyl-p-nitroanalide and its molecular forms using immunoblotting of seminal plasmas of men who were vasectomized or with different sperm concentrations, and in prostatic and seminal vesicle secretions of men undergoing prostatic surgery. Results: DPP IV in seminal plasma of vasectomized men was a membrane associated dimer comprised of subunits of approximately 110 kDa. Its activity did not differ in seminal plasmas of vasectomized, azoospermic, oligozoospermic and normozoospermic men indicating no correlation with the concentration of sperm originally present in the semen. The DPP IV antigen (CD -26) and enzymic activity were present in prostatic secretion, but absent from that of the seminal vesicles. These data indicate that the prostate gland is the primary source of DPP IV activity in seminal plasma. There was little variation in its activities in repeat seminal plasma samples from the same individual, and there was no change in its activity with age to 50 years. Conclusions: DPP IV in seminal plasma was derived from the prostate gland and it may be useful as a bioindicator of prostate function and/or disease with age in men. 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Google Scholar From the Minneapolis VA Medical Center, University of Minnesota Cancer Center, and Departments of Laboratory Medicine and Pathology, Urologic Surgery, Cell Biology and Neuroanatomy, Obstetrics and Gynecology, Genetics and Cell Biology, and Radiology, University of Minnesota, Minneapolis, the Department of Urology, St. Paul Ramsey Medical Center, St. Paul, Minnesota, and the Urologic Research Centre, University of Western Australia, Nedlands, Australia© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited ByWILSON M, HALLER R, LI S, SLATON J, SINHA A and WASSERMAN N (2018) ELEVATION OF DIPEPTIDYLPEPTIDASE IV ACTIVITIES IN THE PROSTATE PERIPHERAL ZONE AND PROSTATIC SECRETIONS OF MEN WITH PROSTATE CANCER: POSSIBLE PROSTATE CANCER DISEASE MARKERJournal of Urology, VOL. 174, NO. 3, (1124-1128), Online publication date: 1-Sep-2005. Volume 160Issue 5November 1998Page: 1905-1909 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information MICHAEL J. WILSON More articles by this author AMY R. RUHLAND More articles by this author JON L. PRYOR More articles by this author CESAR ERCOLE More articles by this author AKHOURI A. SINHA More articles by this author HUGH HENSLEIGH More articles by this author KEITH W. KAYE More articles by this author HUGH J.S. DAWKINS More articles by this author NEIL F. WASSERMAN More articles by this author PRATAP REDDY More articles by this author KHALIL AHMED More articles by this author Expand All Advertisement PDF DownloadLoading ...