Cultivation of Chlorella sorokiniana IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale

Abstract

Flat-panel photobioreactors are effective systems for microalgae cultivation. This paper presents the growth characteristics of the microalgae <i>Chlorella sorokiniana</i> IPPAS C-1 as a result of three-stage scale-up cultivation in a specially designed cultivation system. First, <i>C. sorokiniana</i> was grown aseptically in 250 mL glass vessels; then, it was diluted and inoculated into a 5-liter flat-panel horizontal photobioreactor; and, at the last stage, the culture was diluted and inoculated into a 70-liter flat-panel vertical photobioreactor. In the presented cycle, the cultured biomass increased by 326 times in 13 days (from 0.6 to 195.6 g dw), with a final biomass concentration of 2.8 g dw L^-1. The modes of semi-continuous cultivation were considered. The biomass harvest and dilution of the suspension were carried out either every day or every 3-4 days. For <i>C. sorokiniana</i> IPPAS C-1, a conversion coefficient of optical density values to dry biomass (g L^-1) was refined through a factor of 0.33. The key parameters of the photobioreactors tested in this work are discussed.