Agricultural and Biological Chemistry · 1978 · 84 citations · 0 references
Life SciencesBiochemistryMicrobiologyEnzymologyMycologyPlant BiochemistryEnzyme CharacterizationEnzyme DiscoveryEnzyme InhibitionEnzyme KineticsFungal BiochemistryProteolytic EnzymesCathepsin ActivityCysteine ProteasesElectrophoretic MobilityFungal Enzyme InhibitorsFungal EnzymologyProtease InhibitionProtease InhibitorsThiol Chemistry
A new thiol protease inhibitor, named E–64, was isolated from the extract of a solid culture of Aspergillus japonicus TPR–64 freshly isolated from soil. E–64 was obtained as white needles and the empirical formula was estimated to be C15N5–H27O5 (M. W. 357). This was almost neutral in its electrophoretic behavior and proved to be a specific and strong inhibitor toward thiol protease such as papain and cathepsin B. They combine equimolecularly and irreversibly.