Development of a new Bacillus carboxyl esterase for use in the resolution of chiral drugs

Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.

David A. Estell, Thomas P. Graycar, James A. Wells · Journal of Biological Chemistry · 1985 · 336 citations · Full text

Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers

Patrick Stanssens, Chris Opsomer, Yvonne M. McKeown et al. · Nucleic Acids Research · 1989 · 313 citations · Full text

The family of highly interrelated single-stranded deoxyribonucleic acid plasmids.

Alexandra Gruss, S. Dusko Ehrlich · Microbiological Reviews · 1989 · 213 citations · Full text

Dna Sequencing, Nucleic Acid Chemistry, Natural Sciences +15

An improved positive selection plasmid vector constructed by ollgonucleotide mediated mutagenesis

Björn Nilsson, Mathias Uhlén, Staffan Josephson et al. · Nucleic Acids Research · 1983 · 154 citations · Full text

Use of lipases in the resolution of racemic ibuprofen

Annikka Mustranta · Applied Microbiology and Biotechnology · 1992 · 151 citations

Pharmacology, Lipid Analysis, Racemic Ibuprofen