THE ESTIMATION OF SUBSTITUTED THIOOXAZOLIDONES IN RAPESEED MEALS

Abstract

A quantitative method is described for the separate determination of thiooxazolidone and isothiocyanate in a single sample of rapeseed. The procedure involves first the estimation of the isothiocyanate, which is removed from the macerated meal by steam distillation. Then the filtered residue is shaken with diethyl ether and the quantity of thiooxazolidone in the extract is determined spectrophotometrically at 248 mμ. Recoveries of thiooxazolidone added to rapeseed meal are quantitative and precautions required to give reproducible results are outlined. The thiooxazolidone content of rapeseed meal and also the isothiocyanate/thiooxazolidone ratio vary considerably from sample to sample.