Determination and Comprehensive Risk Assessment of Dietary Exposure to Ochratoxin A on Fermented Teas

Abstract

A specialized method for ochratoxin A (OTA) determination on fermented teas was developed and validated using ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Methodology results showed that recovery, relative standard deviation, accuracy, and precision were qualified. The limits of detection and quantification were 0.32 and 0.96 μg/kg, respectively. Two of 158 collected samples were screened for OTA contamination. Comprehensive risk assessment based on OTA contaminations of this study and other peer-reviewed publications was performed. The highest hazard quotient (HQ) value (8.86 × 10–2) and the highest 1/MoE value (8.61 × 10–5) in probabilistic assessment were equally below the recommended non-neoplastic and neoplastic thresholds, indicating no health risks. However, the HQ and 1/MoE values of the 95th percentiles in 20–39 and ≥50 years of age were close to thresholds of 1.0 and 1.0 × 10–4, respectively. Under the extreme case, there were only a few scenarios (e.g., 40–49 years of age) of HQ values below the non-neoplastic threshold, but the 1/MoE value of each group exceeded the neoplastic threshold. This is the first extensive risk assessment on OTA from fermented teas worldwide, but the sample size is still limited, and a large number of samples is encouraged in a future study for a more accurate assessment.