Four new prenylflavonol glycosides from the leaves of <i>Cyclocarya paliurus</i>

Abstract

Four new prenylflavonol glycosides (<b>1-4</b>) along with two known analogues (<b>5-6</b>) were isolated from the leaves of <i>Cyclocarya paliurus</i> for the first time. The structures of these compounds were characterized by comprehensive analysis of 1 D, 2 D NMR, HRESIMS, UV data and enzymatic hydrolysis. In bioassays, compounds <b>1-4</b> were evaluated for inhibitory effects on xanthine oxidase (XOD) and effects on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS) induced RAW264.7 cells. Moreover, compounds <b>1</b> and <b>2</b> showed outstanding XOD inhibitions with IC₅₀ values of 18.16 ± 3.91 and 37.65 ± 5.67 µM, and exhibited inhibitions against LPS-induced NO production with IC₅₀ values of 80.50 ± 3.09 and 82.28 ± 2.87 µM.