Concepedia

A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers

I. Ayelen Ramallo, Susana Zacchino, Ricardo L. E. Furlán

Phytochemical Analysis · 2005 · 109 citations · 22 references

DOI

Abstract

A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non-enzymatic version of the autographic assay wherein superoxide is chemically generated.

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