An Optimized Protocol for Proximity Biotinylation in Confluent Epithelial Cell Cultures Using the Peroxidase APEX2

Directed evolution of APEX2 for electron microscopy and proximity labeling

Stephanie S Lam, Jeffrey D. Martell, Kimberli J. Kamer et al. · Nature Methods · 2014 · 1.4K citations · Full text

Electron Microscopy, Microscopy, Microscopy Method +7

Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules

Sebastian Markmiller, Sahar Soltanieh, Kari L. Server et al. · Cell · 2018 · 927 citations · Full text

Protein Function, Functional Genomics, Interactomics +11

Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2

Victoria Hung, Namrata D. Udeshi, Stephanie S Lam et al. · Nature Protocols · 2016 · 590 citations · Full text

Proteomic Technology, Biochemistry, Engineered Peroxidase Apex2 +12

Proteomic Mapping of the Human Mitochondrial Intermembrane Space in Live Cells via Ratiometric APEX Tagging

Victoria Hung, Peng Zou, Hyun‐Woo Rhee et al. · Molecular Cell · 2014 · 518 citations · Full text

Ratiometric Apex Tagging, Live Cells, Mitochondrial Function +11

An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells

Braden T. Lobingier, Ruth Hüttenhain, Kelsie Eichel et al. · Cell · 2017 · 417 citations · Full text

Signal Transduction, Engineering, Interactomics +11