Journal of Medicinal Chemistry · 2020 · 40 citations · 36 references
MedicineMedicinal ChemistryPharmacologyDrug DesignDrug DiscoveryMolecular OncologyEnzyme InhibitionOncogenic Signaling PathwaysOncology PharmacologyTyrosine Kinase InhibitorsBiomarkersCancer Cell Proliferation InhibitionCancer Signaling PathwaysDose-dependent SelectivityPoly(adp-ribose) Polymerase InhibitionProtein Kinase InhibitorsTargeted Inhibitor DesignWnt Signaling Modulation
Tankyrases (TNKS/TNKS2) belong to the poly(ADP-ribose) polymerase family. Inhibition of their enzymatic activities attenuates the Wnt/β-catenin signaling, which plays an important role in cancer pathogenesis. We previously reported the discovery of RK-287107, a spiroindoline-based, highly selective, potent tankyrase inhibitor. Herein we describe the optimization process of RK-287107 leading to RK-582, which exhibits a markedly improved robust tumor growth inhibition in a COLO-320DM mouse xenograft model when orally administered. In addition to the dose-dependent elevation and attenuation of the levels of biomarkers AXIN2 and β-catenin, respectively, results of the TCF reporter and cell proliferation studies on COLO-320DM are discussed.
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