Concepedia

Glucocerebrosidases catalyze a transgalactosylation reaction that yields a newly-identified brain sterol metabolite, galactosylated cholesterol

Hisako Akiyama, Mitsuko Ide, Yasuko Nagatsuka, Tomoko Sayano, Etsuro Nakanishi, Norihito Uemura, Kohei Yuyama, Yoshiki Yamaguchi, Hiroyuki Kamiguchi, Ryōsuke Takahashi,

Journal of Biological Chemistry · 2020 · 41 citations · 52 references

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Abstract

enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain.

References

52

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES

Jordi Folch, Marjorie B. Lees, G H S Stanley · Journal of Biological Chemistry · 1957 · 64.9K citations · Full text

, , +11

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

E. G. Bligh, W. J. Dyer · Canadian Journal of Biochemistry and Physiology · 1959 · 48.9K citations

, , +9

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

E. G. Bligh, W. J. Dyer · Canadian Journal of Biochemistry and Physiology · 1959 · 22.9K citations

, , +9

THE COLORIMETRIC DETERMINATION OF PHOSPHORUS

Cyrus H. Fiske, Y. Subbarow · Journal of Biological Chemistry · 1925 · 19.1K citations · Full text

, , +8

Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes

Katarina Trajković, Chieh Hsu, Salvatore Chiantia et al. · Science · 2008 · 3.6K citations · Full text

, , +17