Solid-phase extraction and high performance liquid chromatography with diode array detection method for the determination of antibiotic residues in poultry tissues

Abstract

This article presents information on high performance liquid chromatography with diode array detection (HPLC-DAD) method for the simultaneous determination of six antibiotic residues (enrofloxacin, sulfadimethoxine, sulfamerazine, sulfamethoxazole, sulfamoxole, and tylosin) in three poultry tissues. The target antibiotic residues were extracted from raw poultry samples following concentration, clean-up through solid phase extraction. The data describe the extraction, determination and screening procedures of these common antibiotic residues in 111 samples of fresh and frozen poultry meats. The limit of detection (LOD) ranged from 5.37–55.4 ng/g, while the quantification limit (LOQ) was in the range of 17.9–184 ng/g, respectively, with minimal matrix effect. The calibration curves obtained exhibited a good linear response with the coefficient of determination, R2 > 0.996. Some concentrations exceeded their maximum residue limits in most samples. These findings indicated elevated levels of antibiotic residues in tissues of locally produced and illegally imported poultry meat samples.