PubMed · 2020 · 15 citations · 28 references
Life SciencesCell BiologyImmunologyCell DifferentiationRegenerative MedicineStem Cell BiologyAdipose TissueMesenchymal Stem Cell TherapyRegulatory T CellsStem Cell PhysiologyT Cell BiologyT Cell DifferentiationAdipose Stem CellsAdipose Tissue Immune CellsAdipose-derived Mesenchymal Stem CellsAdipose-derived Mesenchymal Stromal CellsInducible Regulatory T CellsPeripheral Regulatory T Cells
OBJECTIVE: Mesenchymal stem cells (MSCs) have prominent immunomodulatory roles in the tumor microenvironment. The current study intended to elucidate Treg subsets and their cytokines after exposing naïve T lymphocytes to adiposederived MSCs (ASCs). MATERIALS AND METHODS: T cells were then co-cultured with ASCs for five days. The phenotype of regulatory T cells (Tregs) and production of interleukine-10 (IL-10), transforming growth factor beta (TGF-β) and IL-17 were assessed using flow cytometry and ELISPOT assays, respectively. RESULT: Tregs were expanded after co-culturing of T cells with both cancer-ASCs and normal-ASCs, while they were statistically significant only in the presence of cancer-ASCs (P<0.05). Production of IL-10, IL-17 and TGF-β by T cells was increased in the presence of either normal- or cancer-ASCs; however, significant effect was only observed in the IL-10 and TGF-β of cancer-ASCs (P<0.05). CONCLUSION: The results further confirm the immunosuppressive impacts of ASCs on T lymphocytes and direct them to specific regulatory phenotypes which may support immune evasion and tumor growth.
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