Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

TLDR

Guidelines are a consensus effort of many members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry to help immunologists avoid common errors that undermine data. The latest flow cytometry techniques and applications are described, with examples of data generation and analysis. The book includes comprehensive sections on major immune cell types with phenotype tables for murine and human cells, plus expert‑written tips, tricks, and pitfalls, making it an essential research companion.

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.