Gut Microbiota and Metabolome Response of <i>Decaisnea insignis</i> Seed Oil on Metabolism Disorder Induced by Excess Alcohol Consumption

Abstract

This study investigated the modulatory effects of <i>Decaisnea insignis</i> seed oil (DISO), which was rich in palmitoleic acid (55.25%), palmitic acid (12.25%), and oleic acid (28.74%), on alcohol-induced metabolism disorder in mice. Fifty mice were orally administered with 38% alcohol (0.4 mL/day) and without or with DISO (3, 6, and 12 g/kg) for consecutive 12 weeks. DISO inhibited the alcohol-induced weight loss and liver function abnormality (<i>p</i> < 0.01) and shifted the profiles of cecal microbiome: elevating the abundance of <i>Lactobacillus</i>, <i>Ruminoccoceae</i>_<i>UCG</i>_004 (<i>p</i> < 0.05) and decreasing abundance of <i>Parabacteroides</i> (<i>p</i> < 0.05). This treatment also regulated metabolome response of amino acid and lipid metabolism in cecal content: upregulating 5-hydroxyindole-3-acetic acid (<i>p</i> < 0.05), 6-hydroxynicotinic acid, 5-methoxytryptamine, nicotinamide, and nicotinic acid (<i>p</i> < 0.1) and downregulating androsterone, tryptophan, and indole-3-acetamide (<i>p</i> < 0.05). DISO protected against alcoholic liver injury and gut microbiota dysbiosis by enriching the relative abundance of <i>Lactobacillus</i>, which was positively associated with the improvement of intestinal permeability and tryptophan metabolism.