Low genetic variation is associated with low mutation rate in the giant duckweed

Abstract

Abstract Mutation rate and effective population size ( N e ) jointly determine intraspecific genetic diversity, but the role of mutation rate is often ignored. Here we investigate genetic diversity, spontaneous mutation rate and N e in the giant duckweed ( Spirodela polyrhiza ). Despite its large census population size, whole-genome sequencing of 68 globally sampled individuals reveals extremely low intraspecific genetic diversity. Assessed under natural conditions, the genome-wide spontaneous mutation rate is at least seven times lower than estimates made for other multicellular eukaryotes, whereas N e is large. These results demonstrate that low genetic diversity can be associated with large- N e species, where selection can reduce mutation rates to very low levels. This study also highlights that accurate estimates of mutation rate can help to explain seemingly unexpected patterns of genome-wide variation.