G3 Genes Genomes Genetics · 2019 · 86 citations · 14 references
Life SciencesGeneticsMolecular GeneticsGene ExpressionGenetic EngineeringGenome EditingCrispr-based EngineeringCrispr-cas SystemsGene Knock-in StrategiesGene SilencingTarget Site SelectionTransgene Expression ControlC. Elegans Developmental ModelsCrispr-assisted IntegrationCrispr-based Genome EngineeringCrispr-cas Genome EditingCrispr-cas9 Genome EditingCrispr-cas9 Knockout ModelsCrispr-cas9 Mediated Knock-inCrispr-cas9-mediated Repair
Abstract We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans.
14
Single-copy insertion of transgenes in Caenorhabditis elegans
Christian Frøkjær‐Jensen, M. Wayne Davis, Christopher E. Hopkins et al. · Nature Genetics · 2008 · 1.3K citations · Full text
Heritable genome editing in C. elegans via a CRISPR-Cas9 system
Ari E. Friedland, Yonatan B. Tzur, Kevin M. Esvelt et al. · Nature Methods · 2013 · 944 citations · Full text