CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology · 2016 · 5K citations

Life Sciences, Biotechnology, Molecular Biology +13

DNA targeting specificity of RNA-guided Cas9 nucleases

Patrick D. Hsu, David A. Scott, Joshua A. Weinstein et al. · Nature Biotechnology · 2013 · 4.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +10

Vascular endothelial growth factor/vascular permeability factor is temporally and spatially correlated with ocular angiogenesis on a primate model

Miller Jw · UCL Discovery (University College London) · 1994 · 584 citations

Medicine, Ophthalmology, Vision Science +13

A Rapid and General Assay for Monitoring Endogenous Gene Modification

Dmitry Guschin, Adam James Waite, George E. Katibah et al. · Methods in molecular biology · 2010 · 510 citations

Life Sciences, Genetics, Molecular Biology +9

In Vivo Knockout of the Vegfa Gene by Lentiviral Delivery of CRISPR/Cas9 in Mouse Retinal Pigment Epithelium Cells

Andreas Holmgaard, Anne Louise Askou, Josephine Natalia Esther Benckendorff et al. · Molecular Therapy — Nucleic Acids · 2017 · 73 citations · Full text

Life Sciences, Biotechnology, Genetics +17