An improved secretion signal enhances the secretion of model proteins from Pichia pastoris

AGGRESCAN: a server for the prediction and evaluation of "hot spots" of aggregation in polypeptides

Oscar Conchillo‐Solé, Natalia Sánchez de Groot, Francesc Avilés et al. · BMC Bioinformatics · 2007 · 1.1K citations · Full text

Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange ^TM Site-Directed Mutagenesis

Wenyan Wang, Bruce A. Malcolm · BioTechniques · 1999 · 598 citations · Full text

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

Janet Kurjan, Ira Herskowitz · Cell · 1982 · 590 citations

Natural Sciences, Genetics, Molecular Biology +8

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

A J Brake, James P. Merryweather, Daniel G. Coit et al. · Proceedings of the National Academy of Sciences · 1984 · 488 citations · Full text

Protein Secretion, Mature Foreign Proteins, Genetics +23

Signal sequences specify the targeting route to the endoplasmic reticulum membrane.

Davis Ng, Jeremy D. Brown, Peter Walter · The Journal of Cell Biology · 1996 · 458 citations · Full text

Genetics, Signal Recognition, Molecular Biology +22