Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases

Abstract

This study identified <i>Tres</i>Man5A as the best endomannanase for increasing cellulase catalysed glucose release from softwood. Except for the superior performance of <i>Tres</i>Man5A, the fungal GH5 and GH26 endomannanases generally performed on par on the lignocellulosic matrix. The work also illustrated the importance of using genuine lignocellulosic substrates rather than simple model substrates when selecting enzymes for industrial biomass applications.