Metabolic pathway engineering for high-level production of 5-hydroxytryptophan in Escherichia coli

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15K citations · Full text

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods · 2009 · 10.6K citations

Dna Nanotechnology, Dna Sequencing, Dna +9

Automated design of synthetic ribosome binding sites to control protein expression

Howard M. Salis, Ethan A. Mirsky, Christopher A. Voigt · Nature Biotechnology · 2009 · 1.9K citations · Full text

Engineering, Protein Expression, Protein Folding +14

Synthesis of Serotonin by a Second Tryptophan Hydroxylase Isoform

Diego J. Walther, Jens‐Uwe Peter, Saleh Bashammakh et al. · Science · 2003 · 1.5K citations

Neurotransmitter, Psychopharmacology, Enzymatic Modification +18

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

Jing Du, Yongbo Yuan, Tong Si et al. · Nucleic Acids Research · 2012 · 239 citations · Full text

Engineering, Molecular Biology, Synthetic Circuit +17