Integrated ferroelectrics · 2017 · 13 citations · 22 references
EngineeringBiochemistryBiomedical EngineeringBiocompatibilityBiomaterialsTissue EngineeringBio-based MaterialsBiodegradable BiomaterialsBiomaterial CharacterizationBiomaterials For RegenerationBiomaterials For Tissue EngineeringProtein AnalysisBiocompatibility Of Protein BiomaterialsBiodegradation Of Protein BiomaterialsDenaturationKeratin BiomaterialsMolecular Weight DeterminationThermal Degradation ProductsThermal Degradation StudiesTissue Regeneration
The Goat Hoof Keratin (GHK) is a valuable biodegradable substance for making tissue regeneration. The protein substance from GHK powder is extracted in a clean form by using modified shindai solution technique. Electrophoreical analysis produces polypeptide chains of molecular weight of 40–45 kDa using SDS-PAGE method. FTIR spectra show the characteristic absorption band for α-helix and β-sheet peptide bonds. DSC analysis provides the GHK denaturation occurred at temperature ranging from 200–250°C and thermal degradation occurs at 211°C & 387°C using TGA. The biocompatibility of GHK examined using MTT (3(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of fibroblast cells shows 91% cell viability.
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