Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

Jeremy J. Agresti, Eugene Antipov, Adam R. Abate et al. · Proceedings of the National Academy of Sciences · 2010 · 1.2K citations · Full text

Engineering, Biochemistry, Biotechnology +17

Determining the optimal PDMS–PDMS bonding technique for microfluidic devices

Mark A. Eddings, Michael Johnson, Bruce K. Gale · Journal of Micromechanics and Microengineering · 2008 · 558 citations

Engineering, Materials Engineering, Materials Science +17

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

Pierre-Yves Colin, Bálint Kintses, Fabrice Gielen et al. · Nature Communications · 2015 · 284 citations · Full text

Life Sciences, Biochemistry, Microbiology +16

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

Fabrice Gielen, Raphaëlle Hours, Stéphane Emond et al. · Proceedings of the National Academy of Sciences · 2016 · 275 citations · Full text

Engineering, Bioengineering, Biotechnology +13

Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

Yolanda Schaerli, Robert C. R. Wootton, Tom Robinson et al. · Analytical Chemistry · 2008 · 260 citations

Life Sciences, Biotechnology, Molecular Biology +17