Multilevel engineering of the upstream module of aromatic amino acid biosynthesis in Saccharomyces cerevisiae for high production of polymer and drug precursors

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods · 2009 · 10.9K citations

Life Sciences, Biochemistry, Molecular Biology +16

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Carola Engler, Romy Kandzia, Sylvestre Marillonnet · PLoS ONE · 2008 · 2.6K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.

Cathie T. Chung, Sanna‐Mari Niemelä, R.H. Miller · Proceedings of the National Academy of Sciences · 1989 · 1.6K citations · Full text

Life Sciences, Biotechnology, Microbiology +9

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

Tessa G. Montague, José Manuel Bautista Santa Cruz, James A. Gagnon et al. · Nucleic Acids Research · 2014 · 1.3K citations · Full text

Life Sciences, Biotechnology, Genetics +16

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia et al. · PLoS ONE · 2009 · 1.1K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17