Concepedia

High-throughput purification of recombinant proteins using self-cleaving intein tags

Michael J. Coolbaugh, Miriam J. Shakalli Tang, David W. Wood

Analytical Biochemistry · 2016 · 18 citations · 20 references

DOI

Concepts

References

20

A genetic system yields self-cleaving inteins for bioseparations

David W. Wood, Wei Wu, Georges Belfort et al. · Nature Biotechnology · 1999 · 275 citations

, , +6

His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors

Esmeralda Woestenenk, M. Hammarstrom, Susanne van den Berg et al. · Journal of Structural and Functional Genomics · 2004 · 188 citations

, , +17

Novel and economical purification of recombinant proteins: Intein‐mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association

Mahmoud Reza Banki, Tillman U. Gerngross, David W. Wood · Protein Science · 2005 · 140 citations · Full text

, , +17

Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor

David W. Wood, Victoria Derbyshire, Wentao Wu et al. · Biotechnology Progress · 2000 · 95 citations

, , +17

Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein

M. Hammarstrom, Esmeralda Woestenenk, Niklas Hellgren et al. · Journal of Structural and Functional Genomics · 2006 · 89 citations

, , +16