An enzyme from rat liver catalysing conjugations with glutathione. 2. Replacement of nitro groups

Abstract

2-methylpropan-2-ol-4 25N-ammonia (4:1, v/v). Many solvent systems for these compounds have been proposed (see, for example, Kubota, 1961); the information is given because the system seems to be a very versatile one. It was used (Vining & Taber, 1957) for oc-amino acids and c-hydroxy acids, and later (Russell, 1960) for N-methyl-amino acids. It is a stable one-phase system, and gives compact spots with DNP-derivatives, the R.