Color reaction between pyrogallol red-molybdenum(VI) complex and protein.

Abstract

A color reaction between pyrogallol red(PR) -molybdenum(VI) complex and protein was investigated. A spectrophotometric method using the PR-molybdenum(VI) complex could be used in the concentration range of 0 - 400 μg/10 ml of albumin, where the Sandell sensitivity was calculated to be 0.0048 μg/cm2 for albumin at 600 nm. A detection test of protein on a spot plate was also studied. The proposed method was applied to the determination of urinary protein; analytical results were satisfactory.