Isolation, culture and transplantation of female germline stem cells from neonatal and prepubertal mice

Abstract

The successful isolation and culture of female germline stem cells \(FGSCs) that exist in postnatal and adult mammals will allow us to study their biological characteristics and their applications in biotechnology and medicine. In this study, we describe systematic procedures for the culture and transplantation of FGSCs. To establish mouse FGSC lines, the FGSCs were isolated from the ovaries of postnatal mice by enzymatic digestion and immunomagnetic puri cation. After culture, FGSCs were transplanted into the ovaries of recipient females that were sterilized by chemotherapy to analyze physiological function. Establishment of FGSC lines allows new possibilities for the use of FGSCs in biotechnology and medicine.