Preparation of therapeutic phospholipids through porcine pancreatic phospholipase A₂‐mediated esterification and lipozyme‐mediated acidolysis

Abstract

Abstract Highly unsaturated fatty acid‐containing phospholipids (HUFA‐PL), which serve to increase the deformability of human red blood cells, were prepared through porcine phospholipase A 2 ‐mediated esterification of the lysophosphatidylcholine, which was derived from soy phosphatidylcholine (PC), and by Lipozyme‐mediated acidolysis. Through these processes, phospholipase A 2 , with formamide as a water mimic, enhanced the incorporation of HUFA into position sn ‐2 of PC and suppressed hydrolysis of the synthesized PL. On the other hand, Lipozyme‐mediated acidolysis between position sn ‐1 of soy PC and HUFA was enhanced by a combination of water and propylene glycol. Simultaneously, the recovered PL products showed decreased hydrolysis of newly synthesized health‐beneficial HUFA‐PL.