A Method of Detection of Fumonisins in Corn Samples Associated with Field Cases of Equine Leukoencephalomalacia

Abstract

Fumonisin B1 and B2, members of a new class of mycotoxins, were measured in culture material of Fusarium moniliforme MRC826, and in two corn samples associated with field cases of equine leukoencephalomalacia. The compounds were detected by thin-layer chromatography (TLC) with confirmation by liquid secondary ion-mass spectrometry (SIMS) and by gas chromatography/mass spectrometry (GC/MS). Reference standards were isolated from cultures of F. moniliforme on corn. The level of fumonisin B1 was about 600 mg/kg in the culture material and 150 and 20 mg/kg in the two naturally contaminated samples.