A rapid and sensitive profiling of free fatty acids using liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) after chemical derivatization

Abstract

A mass spectrometry method for the improved identification and quantification of free fatty acids (FFAs) based on derivatization using trimethylsilyldiazomethane (TMSD) was developed and validated to be an sensitive and accurate method for analyzing FFAs.