A quinone mediator drives oxidations catalysed by alcohol dehydrogenase-containing cell lysates

Abstract

Spontaneous electron transport to molecular oxygen led to regeneration of oxidised nicotinamide cofactor in cell lysates that contain an alcohol dehydrogenase, a quinone reductase and a quinone mediator. This concept allows the efficient oxidation of alcohols in the presence of alcohol dehydrogenase-containing E. coli lysates and catalytic amounts of the quinone lawsone.