Biological Chemistry Hoppe-Seyler · 1996 · 151 citations · 19 references
Public HealthImmunologyDiagnostic MicrobiologyHiv PreventionSalivary DiagnosticsAnalytical ValidationHiv DiagnosisHiv TestingSaliva CollectionAntigen-antibody Hiv TestsAssayCapture Antibody AssaysImmunoassays For SalivaSaliva DiagnosticsSaliva Specimen CollectionSaliva-based DiagnosticsSaliva-based Screening
Novel angiotensin-I-converting enzyme (ACE) inhibitory activities were detected in synthetic peptides corresponding to sequences of beta-lactoglobulin and alpha-lactalbumin and which are known to possess opioid activity. Using hippuryl-histidyl-leucine as substrate, the tetrapeptides beta-lactorphin (Tyr-Leu-Leu-Phe), alpha-lactorphin (Tyr-Gly-Leu-Phe) and beta-lactotensin (His-Ile-Arg-Leu) were shown to have IC50 values of 171.8, 733.3 and 1153.2 microM, respectively. Related dipeptides also inhibited ACE, with Tyr-Leu being the most potent, having an IC50 value of 122.1 microM.
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Plasmid screening at high colony density
Douglas Hanahan, Matthew Meselson · Gene · 1980 · 1.1K citations