Rapid addition of unlabeled silent solubility tags to proteins using a new substrate-fused sortase reagent

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Computing, Computer Science, Computer Systems +14

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Life Sciences, Biochemistry, Molecular Biology +12

<i>Staphylococcus aureus</i> Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall

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Life Sciences, Microbiology, Molecular Biology +11

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Chu di Guana, Ping Lib, Paul D. Riggsa et al. · Gene · 1988 · 675 citations

Life Sciences, Biotechnology, Molecular Biology +9

A general strategy for the evolution of bond-forming enzymes using yeast display

Irwin Chen, Brent M. Dorr, David R. Liu · Proceedings of the National Academy of Sciences · 2011 · 590 citations · Full text

Life Sciences, Biochemistry, Biotechnology +17