Free Radical Research · 2015 · 99 citations · 29 references
MedicineImmunologyPharmacologyImmunopathologyInflammationInnate ImmunityAcute InflammationAcute Lung InjuryAcute Respiratory Distress SyndromeInflammatory Cell ResponsesInflammatory SignalingInflammatory Tissue InjuryAcute Lung Injury PathophysiologyInfiltrationInflammatory Lung InjuryPretreatmentProtective TreatmentsPulmonary Epithelial InjuryPulmonary Inflammatory InjuryTissue Infiltration By Inflammatory Cells
The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40 mg/kg effectively attenuated LPS-induced lung histopathological change, myeloperoxidase or MPO activity, inflammatory cells infiltration, pulmonary wet-to-dry weight ratio, and the generation of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in vivo and in vitro. Furthermore, we demonstrated that ES blocked the activation of NF-кB and RhoA/Rho kinase pathways in LPS-induced mice and A549 cells. The results suggested that ES exhibited protective effect on ALI and might attribute partly to the inhibition of NF-кB and RhoA/Rho kinase pathways in vivo and in vitro.
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Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment
Jianjian Shi, Xiangbing Wu, Michelle Surma et al. · Cell Death and Disease · 2013 · 233 citations · Full text