Myofibroblasts correlate with lymphatic microvessel density and lymph node metastasis in early-stage invasive colorectal carcinoma.

Abstract

BACKGROUND: Recent studies have shown that the interactions between tumor cells and stromal cells are important in tumor development. A possible correlation between tumor-activated myofibroblasts, the main component cells of tumor stroma, and lymphatic microvessel density (LMVD) or other clinical parameters in carcinoma was investigated. MATERIALS AND METHODS: Immunohistochemical examination of alpha-smooth muscle actin and podoplanin were performed in 83 cases of early-stage invasive colorectal carcinoma. RESULTS: There was a good correlation between proliferation of myofibroblasts (PMpt) and LMVD (LMVDpt) in the peri-tumoral area (p = 0.0034). Increased PMpt was also associated with lymphatic invasion (p = 0.0051) and with lymph node metastasis (p = 0.011). However, proliferation of myofibroblasts in intra-tumoral (PMit) areas was not associated with these clinical parameters. CONCLUSION: Proliferation of myofibroblasts in peri-tumoral areas seem to play an important role in lymphangiogenesis, and is also associated with lymph node metastasis.