Statistical analysis on stress induced lipid accumulation along with the major cell components of Chlorella sp.

Abstract

A sequential optimization based on statistical design was employed to optimize the different stress conditions of autotrophic, heterotrophic and mixotrophic levels for the enrichment of lipid in fresh water algae Chlorella sp. The selected individual parameters such as initial pH level, glucose concentration and KNO3 concentration of the medium were optimized by the central composite design under response surface methodology. The maximum lipid content of 49.8% was achieved at the mixotrophic growth condition at 5 g/L of glucose concentration, 3 g/L of KNO3 concentration and pH 7.6 amidst the considered different growth conditions such as autotrophic, heterotrophic and mixotrophic levels comprising along with that of the different individual stress parameters. Similarly maximum protein and chlorophyll yields were achieved at the mixotrophic level with high nitrogen content.