Development and Validation of an RP-HPLC Method for Methionine, Cystine and Lysine Separation and Determination in Corn Samples

Abstract

Corn (Zea mays L.) is a worldwide grown plant which has a high productive potential, twice as much compared to other cereals. It is a major component of the feed formulations for farm animals. Like in the other cereals, the feeding value of the corn protein is determined by the presence of essential amino acids (lysine, methionine, cystine and others). The determination of the essential amino acids concentration from corn is a key-instrument for the nutritionists, irrespective of the animal species. Hence, the necessity to develop more accurate and precise methods to determine the amino acids from the corn used in animal diets.